Detailed Information on Protocols and Resources

Detailed Information on Protocols and ResourcesLukas C. Bossert2023-01-05T19:22:01+01:00

CRC 1382 aims at generating reproducible scientific results in a transparent manner.

In this context, according to the FAIR principles (findable, accessible, inter-operable, re-usable) and under the umbrella of NFDI4Microbiota, project Q02 provides clear information on the work we are doing. Below please find text protocols, links to our resources and tools, and educational videos (generated in collaboration with the AVMZ team; Audiovisual Media Center, University Hospital of RWTH Aachen).

Protocols

Resources and Tools

Github lab repository: https://github.com/ClavelLab
miBC collection: www.dsmz.de/miBC
PiBAC collection: https://www.dsmz.de/PiBAC
ChiBAC collection: https://www.dsmz.de/ChiBAC
Protologger, facilitates the description of novel bacteria: http://www.protologger.de
Integrated Microbial Next Generation Sequencing platform: https://www.imngs.org
RHEA, for analysis of 16S rRNA gene amplicon profiles: https://lagkouvardos.github.io/Rhea

Video Instructions

Step 1: Sample Collection

Step 2: DNA Extraction

Step 3: Amplicon libraries

Step 4: Sequencing Run Preperation