Detailed Information on Protocols and Resources

CRC 1382 aims at generating reproducible scientific results in a transparent manner.

In this context, according to the FAIR principles (findable, accessible, inter-operable, re-usable) and under the umbrella of NFDI4Microbiota, project Q02 provides clear information on the work we are doing. Below please find text protocols, links to our resources and tools, and educational videos (generated in collaboration with the AVMZ team ; Audiovisual Media Center, University Hospital of RWTH Aachen).

Protocols

Resources and Tools

Human intestinal bacterial collection (HiBC): https://www.hibc.rwth-aachen.de
Mouse intestinal bacterial collection (miBC): https://www.dsmz.de/miBC
Pig intestinal bacterial collection (PiBAC): https://www.dsmz.de/PiBAC
Chicken intestinal bacterial collection (ChiBAC): https://www.dsmz.de/ChiBAC
Github lab repository: https://github.com/ClavelLab
Protologger, facilitates the description of novel bacteria: https://www.protologger.de
RHEA, for analysis of 16S rRNA gene amplicon profiles: https://lagkouvardos.github.io/Rhea
Integrated Microbial Next Generation Sequencing platform: https://www.imngs.org

Video Instructions

These videos aim to illustrate major steps of our protocols to sequence or cultivate microbial communities from stool samples. The videos were generated in-house in collaboration with the AVMZ team (Audiovisual Media Center, University Hospital of RWTH Aachen).